GC-MS Assay to Alkaloid Extract of T. foenum-graecum Seeds and Detection Effect of Extract as Antibacterialect Of Extract As Antibacterial
DOI:
https://doi.org/10.32792/utq/utjsci/v11i2.1248Keywords:
antibacterial ,Trigonella foenumAbstract
The need for standardized modern analytical procedures becomes urgent due to the rise in drug-resistant for pathogens. These procedures use to isolate new bioactive compounds from medicinal plants. The medicinal plant-derived compounds could provide a new method to fight pathogenic bacteria. This study sheds light on the antimicrobial activity of plant-derived components, their possible mechanisms of action, and their chemical potential. Five different plant extract concentrations were used:12.5, 25, 50, 100, and 200 mg/ ml. The maximum inhibition zone for the alkaloid extracted from seeds of Trigonella foenum against Klebsiella pneumonia was (21.0 ± 0.63) mm at 200 Mg ml1 dose. In comparison, the minimum inhibition zone for Pseudomonas aeruginosa was (6.16 ± 0.40) mm at 25 Mg ml1. According to the results of the gas chromatography-mass spectrometry (GC-MS) analysis, the chemical composition analysis of the Trigonella foenum extract had a high concentration of organic components with antimicrobial and antioxidant properties.
Received: 2024-05-08
Revised: 2024-05-24
Accepted: 2024-10-06
References
1-[1] R. A. A. L. Askary, “Effectiveness study of Artemisia herba-alba and Borage officinalis leaf extract against bacteria Staphylococcus aureus,” Univ. Thi-Qar J. vol. 8, no. 1, pp. 113-117, 2021
H. Archana and V. G. Bose, “Evaluation of phytoconstituents from selected medicinal plants and its synergistic antimicrobial activity,” Chemosphere, Vol. 8, no. 1. Pp. 113-117, 2022.
S. Wang, X.-Q. Liu, O. Kang, and D. Kwon, “Combination of Sanguisorbigenin and Conventional Antibiotic Therapy for Methicillin-Resistant Staphylococcus aureus: Inhibition of Biofilm Formation and Alteration of Cell Membrane Permeability,” International Journal of Molecular Science, Vol. 23, no. 8. Pp. 4220-4232, 2022.
H. A. H. Alsaidy and A. A. Ali, “Reviews on Plants Gum and Mucilage I: The Composition and Pharmacological Significance of Turmeric (Curcuma longa) Rhizomes Polysaccharides and Mucilage/their Aqueous Extracts Commercial Products,” Univ. Thi-Qar J. Sci., vol. 8, no. 1, pp. 14–25, 2021.
H. Huang et al., “The Effects of Fenugreek Extract on Growth Performance, Serum Biochemical Indexes, Immunity and NF-κB Signaling Pathway in Broiler,” Frontiers in Veterinary Science, Vol. 9, no. 6. Pp. 882754, 2022.
M. S. Akhtar, M. K. Swamy, and U. R. Sinniah, "Natural bio-active compounds," Natural Bio-Active Compounds, vol. 1, pp. 1-608, 2019.
R. Prasad, S. Acharya, and J. Thomas, “Identification of High Seed Yielding And Stable Fenugreek Mutants” by | Perlego,” 6. 01, 2014.
B. Bin-Hafeez, R. Haque, S. Parvez, S. Pandey, I. Sayeed, and S. Raisuddin, “Immunomodulatory effects of fenugreek (Trigonella foenum graecum L.) extract in mice,” International Immunopharmacology, Vol. 12, no. 4. Pp, 257-265, 2003.
L. a N. Al-Timimi, “Antibacterial and Anticancer Activities of Fenugreek Seed Extract,” Asian Pacific Journal of Cancer Prevention, Vol. 20, no. 12. Pp, 3771, 2019.
T. H. Desai and S. V. Joshi, “Anticancer activity of saponin isolated from Albizia lebbeck using various in vitro models,” Journal of Ethnopharmacology, Vol. 231, no. 6. Pp, 494-502, 2019.
G. K. Devi, K. Manivannan, G. Thirumaran, F. A. A. Rajathi, and P. Anantharaman, “In vitro antioxidant activities of selected seaweeds from Southeast coast of India,” Asian Pacific Journal of Tropical Medicine, Vol. 4, no. 3. Pp, 205-211, 2011.
Ch. Romay, R. Gonzalez, N. Ledon, D. Remirez, and V. Rimbau, “C-Phycocyanin: A Biliprotein with Antioxidant, Anti-Inflammatory and Neuroprotective Effects,” Current Protein and Peptide Science/Current Protein & Peptide Science, Vol. 4, no. 3. Pp, 207-216, 2003.
M. Nafees, S. Ullah, Barkatullah, A. Javaid, and Shumaila, “PHARMACOGNOSTIC AND PHYTOCHEMICAL SCREENING OF COCCULUS PENDULUS Diels. STEM AND ROOT,” Vol. 30, no. 5. Pp, 114-126, 2020.
R. Yadav and M. Agarwala, “Phytochemical analysis of some medicinal plants,” Vol. 3, no. 12. Pp, 10-14, 2011.
A. A. S. Abdul, A. O. Abbas, and B. N. Abdulhadi, “Comparative study for antibiotic susceptibility against Pseudomonas aeruginosa isolated from otitis media through several years in Thi-Qar Province/Iraq." Journal of Univesity of Thi-Qar | EBSCOhost,” openurl.ebsco.com, Vol. 16, no. 3. Pp, 33-43,2021.
N. Bobby, E. Wesely, and M. Johnson, “High performance thin layer chromatography profile studies on the alkaloids of Albizia lebbeck,” Vol. 2, no. 1. Pp, 1-6, 2012.
“Mass Spectrometry Data Center, NIST.” https://chemdata.nist.gov/
“Phytochemical Methods,” SpringerLink. https://link.springer.com/book/10.1007/978-94-009-5570-7
E. Mezal, Z. K. Hanan, and M. Saleh, “IDENTIFICATION, ANTIMICROBIAL RESISTANCE OF SALMONELLA ENTERICA ISOLATED FROM DIARRHEAL CHILDREN IN THI-QAR PROVINCE DURING 2015, Thesis” 2016.
“WHO model list of essential medicines, 20th list (March 2017, amended August 2017),” 2017. https://iris.who.int/handle/10665/273826
C. Jain, S. Khatana, and R,Vijayvergia, “Bioactivity of secondary metabolites of various plants: A review,” Int. J. Pharm. Sci. Res., vol. 10, no. 2, pp. 494–504, 2019.
Indriati et al., “GC-MS and Antioxidant Capacity Analysis in Propanol Extract of Carthamus tinctorious L,” Journal Peneitan, vol. 8, no. 1, pp. 67–73, 2021.
T. Tyagi and M. Agarwal, “Phytochemical screening and GC-MS analysis of bioactive constituents in the ethanolic extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) solms,” www.phytojournal.com, vol. 6, no. 1, pp. 195–206, vol. 10, no. 1, pp. 17–26, 2017.
L. C. Pal, S. Agrawal, A. Gautam, J. K. Chauhan, and C. V. Rao, “Hepatoprotective and Antioxidant Potential of Phenolics-Enriched Fraction of Anogeissus acuminata Leaf against Alcohol-Induced Hepatotoxicity in Rats,” Medical Sciences, vol. 10, no. 1, pp. 17–31, 2022.
A. A. Adenike, P. Adegbola, and O. S. Fadahunsi, "Antioxidant property and GCMS profile of oil extracted from Cocos nucifera using a fermentation method," BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology, vol. 100, 2019.
G. E. Egbung, C. Anosike, A. B. Utu-Baku, I. Ogar, and V. U. Nna, "Phytochemical evaluation and GC-MS analysis of Hyptis verticillata cultivated in Calabar Cross River State, Nigeria," International Journal of Biological and Chemical Sciences, vol. 11, pp. 2548-2559, 2017.
V. Kumar and Y. Sharma, “Effects of environment on the chemical constituents and biological characteristics of some medicinal plants,” Apple Academic Press eBooks, Dec. 12, pp. 279–292, 2018.
L. Salerno et al., “Antioxidant Activity and Phenolic Content of Microwave-AssistedSolanum melongenaExtracts,” the Scientific World Journal/TheScientificWorldjournal, vol. 20, no. 8, pp. 1–6, 2014
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