Isolation and identification of Streptococcus pneumoniae that causes Respiratory Diseases

Authors

  • Al–Jowrani, S. H. Department of Biology, College of Science, University of Thi-Qar , Thi-Qar,Iraq.
  • Al-Mozan, H. D. K. Department of Biology, College of Science, University of Thi-Qar , Thi-Qar,Iraq.

DOI:

https://doi.org/10.32792/utq/utjsci/v12i1.1366

Keywords:

Streptococcus pneumoniae , VITEK ® 2 compact , Antibiotic resistance

Abstract

Streptococcus pneumoniae is one of the bacterial species that colonize the respiratory system, and it causes many respiratory diseases, including pneumonia. Sputum specimens were collected from 270 patients of all ages suffering from respiratory diseases at the Consultants Clinic for Respiratory and Chest Diseases, Al-Hussein Teaching Hospital, and Public Health Laboratory in Thi-Qar Governorate during the period from October 2024 to March 2025.The aim in this study is the isolation and identification of Streptococcus pneumoniae, and morphological and (conventional and advanced) biochemical tests were performed. Results showed that Streptococcus pneumoniae isolates that cause respiratory diseases were (3.72%). Streptococcus pneumoniae had high rates of resistance to some antibiotics.This study showed that vancomycin is the best to treat Streptococcus pneumoniae, but S. pneumoniae is characterized by high resistance to tetracycline.

References

D. Elahe, "An Overview of Bacterial Respiratory Tract Infections and their Etiologies," Journal of Medical Bacteriology 11, no. 1-2 : 36-46,2023.

L. Ceballos, M. Camila, D. Marin-Palma, W. Zapata, and J. C. Hernandez, "Viral respiratory infections and air pollutants," Air Quality, Atmosphere & Health 15, no. 1: 105-114,2022.

H. Ahmed, and S. Bhatia, "Epidemiology Respiratory Infections: Types, Transmission, and Risks Associated with Co-Infections," In Role of Essential Oils in the Management of COVID-19, pp. 7-17. CRC Press, 2022.

S. Karthik, B. Henriques‐Normark, and S. Normark,"Emerging concepts in the pathogenesis of the Streptococcus pneumoniae: from nasopharyngeal colonizer to intracellular pathogen," Cellular microbiology 21, no. 11 : e13077,2019.

H. Kareema & A. Hanna , "Diagnosis of Vibrio cholerae isolated from patients with Vibriosis in Thi-Qar province," University of Thi-Qar Journal of Science, Dec 5;11(2):8-12, 2024.

A. R. Mohsen, and H. D. K. Al-Mozan, "Isolation and identification of Gram negative bacteria that cause diarrhea, " University of Thi-Qar Journal of Science 10, no. 1 : 175-180,2023.

S. J. Young, B. W. Eun, and M. H. Nahm, "Diagnosis of pneumococcal pneumonia: current pitfalls and the way forward," Infection & chemotherapy 45, no. 4 : 351-366,2013.

W. O. Daningrat., W. T. Paramaiswari, H. F. M. Putri, and D. Safari, "Antimicrobial resistance profile of Streptococcus pneumoniae in children< 5 years of age in sea nomads population in Indonesia," International Journal of Infectious Diseases 101 : 13, 2020.

A. k. Asaad, N. Soleimani, and H. Mousa, "Investigation of Antimicrobial Susceptibility Patterns and blaVIM-metallo-β-lactamase Gene in Clinical Samples of Klebsiella pneumoniae," University of Thi-Qar Journal of Science 10, no. 2: 242-246, 2023.

A. H. D. KHALAF, "Molecular diagnosis of Pseudomonas aeruginosa isolated from clinical and milk samples," Biodiversitas Journal of Biological Diversity 25, no. 8 ,2024.

L. Michael J., B. E. Pierce. A photographic atlas for the microbiology laboratory 3rd Edition. Morton Publishing Company; 2005.

VITEK® 2: Healthcare | bioMérieux – bioMerieux, Inc [Internet].

P. Ioannis, S. Depickère, T. Braeye, M .Mukovnikova, A. Vodolazkaia, C. Abels, L. Cuypers et al, "Non-invasive Streptococcus pneumoniae infections are associated with different serotypes than invasive infections, Belgium, 2020 to 2023," Eurosurveillance 29, no. 45 : 2400108,2024.

F. M. Laura, F. Coghe, A. Scano, M. Carta, and G. Orrù, "Co-infection of Streptococcus pneumoniae in respiratory infections caused by SARS-CoV-2," Biointerface Research in Applied Chemistry 11, no. 4 : 12170-12177,2021.

C. Richard, "Gram staining," Current protocols in microbiology 1 , A-3C, 2006.

S. Mahato ., H. K Sah, and S. Yadav, "Isolation of Streptococcus pneumoniae from the sputum samples and their antimicrobial resistance in Biratnagar, Nepal," J Microbiol Exp 7, no. 6 (2019): 299-304.

D. Greenwood ., R. C. B. Slack, and J. F. Peutherer, "Medical microbiology. A guide to microbial infections: pathogenesis, immunity, laboratory diagnosis and control," xiii+-690, 2012

K. Tran-Quang, N .Thi-Dieu Thuy, T.D .Hung, P. H. Van, N. V. Trung, T. X. Bach, L. Mattias, and D. Q. Sy, "Antibiotic resistance of Streptococcus pneumoniae in Vietnamese children with severe pneumonia: a cross-sectional study" ,20231622506, 2023.

F . Alister J., and A. P. Davis, "Vancomycin mimicry: Towards new supramolecular antibiotics," Organic & biomolecular chemistry 20, no. 39 : 7694-7712, 2022.

K. H. Benyiam, M. M. Ali, and M. D. Ormago, "Prevalence and Multi-Drug Resistance of Streptococcus pneumoniae Infection Among Presumptive Tuberculosis Adult Cases at Dilla University Referral Hospital, Dilla, Ethiopia," Infection and Drug Resistance : 5183-5191, 2022.

H. Angela, J. Bielicki, M. N. Clements, N. Frimodt-Møller, A. E. Muller, J.P. Paccaud, and J. W. Mouton, "Oral amoxicillin and amoxicillin–clavulanic acid: properties, indications and usage, " Clinical Microbiology and Infection 26, no. 7: 871-879, 2020.

M. Zahraa Y., and H. S. Naher, "Antimicrobial susceptibility of Streptococcus pneumoniae isolates causing LRTI in Najaf, Iraq," Environmental & Socio-economic Studies 5, no. 2 : 10-18, 2017.

H. Z. Rashid, A. S. Motib, and A. F. Abbas, "Adaptability of Biofilm Formation in Streptococcus Pneumoniae to Various Growth Conditions," Indian Journal of Forensic Medicine & Toxicology 15, no. 2 , 2021.

Downloads

Published

2025-06-27

Issue

Section

Articles

Categories

How to Cite

AL Jowrani, S. H. H. ., & Al-Mozan , H. D. K. . (2025). Isolation and identification of Streptococcus pneumoniae that causes Respiratory Diseases. University of Thi-Qar Journal of Science, 12(1), 189-194. https://doi.org/10.32792/utq/utjsci/v12i1.1366