Assessment of IL-27 as a Predictive Biomarker for Immune Response in Asthmatic Patients in Diyala Governorate
DOI:
https://doi.org/10.32792/utq/utjsci/v12i1.1368Keywords:
Allergy, Asthma, IL-27, CRP, Total IgEAbstract
Asthma is a persistent inflammatory respiratory disease characterized by coughing, wheezing, and intermittent dyspnea, as well as a sensation of chest tightness. The relationship between interleukin-27 (IL-27) and asthma is not precisely known yet. This study attempted to estimate the serum level of IL-27 and its relationship with immunological and inflammatory markers in patients suffering from asthma. The demographic characteristics of this study were analyzed statistically. The serum levels of IL-27 were evaluated by Enzyme-linked immunosorbent assay (ELISA). CRP and Total IgE were evaluated by Cobas C111 and Cobas E411, respectively. The study showed a highly significant difference in IL-27, CRP, and total IgE when we compared between the patient group (62.12 ± 16.58 pg/ml, 11.2 ± 6.29 mg/l, and 297.26 ± 86.6 IU/ml), and the healthy control group (39.32 ± 3.576 pg/ml, 2.49 ± 1.1 mg/l, and 93 ± 18.3 IU/ml) respectively. ROC analysis of the studied biomarkers showed high sensitivity and specificity. That observation of elevation in all studied biomarker levels in asthma patients indicates a strong evidence in the diagnosis of asthma, as well as in the differentiation between acute and chronic cases, and in determining the severity of asthma.
Received: 2025-03-30
Revised: 2025-04-22
Accepted: 2025-05-07
References
P. Soccio et al., "Breath and Sputum Analyses in Asthmatic Patients: An Overview," Cells, vol. 13, no. 16, p. 1355, 2024. [Online]. Available: https://www.mdpi.com/2073-4409/13/16/1355.
V. Gonzalez-Uribe, S. J. Romero-Tapia, and J. A. Castro-Rodriguez, "Asthma Phenotypes in the Era of Personalized Medicine," J Clin Med, vol. 12, no. 19, p. 6207, Sep 26 2023, doi: 10.3390/jcm12196207.
S. AlBloushi and M. Al-Ahmad, "Exploring the immunopathology of type 2 inflammatory airway diseases," (in English), Front Immunol, Review vol. 15, p. 1285598, 2024-April-12 2024, doi: 10.3389/fimmu.2024.1285598.
W. D. Xu, D. C. Wang, M. Zhao, and A. F. Huang, "An updated advancement of bifunctional IL-27 in inflammatory autoimmune diseases," Front Immunol, vol. 15, p. 1366377, 2024, doi: 10.3389/fimmu.2024.1366377.
U. A. Khanam, D. C. Rennie, K. Davis, and J. A. Lawson, "Are Dietary Factors Associated with Lung Function in Canadian Adults?," Can J Diet Pract Res, vol. 81, no. 1, pp. 28-36, Mar 1 2020, doi: 10.3148/cjdpr-2019-023.
J. Qasim et al., "High-Sensitivity C -reactive protein Assessment in Bronchial Asthma: Impact of Exhaled Nitric Oxide and Body Mass Index," Systematic Reviews in Pharmacy, vol. 11, p. 705, 05/08 2020, doi: 10.31838/srp.2020.3.26.
M. O. Al Ghobain et al., "The correlation between fractional exhaled nitric oxide (FeNO), blood eosinophil count, immunoglobulin E levels, and spirometric values in patients with asthma," Cureus, vol. 15, no. 2, 2023.
T. A. E. Platts-Mills, A. J. Schuyler, E. A. Erwin, S. P. Commins, and J. A. Woodfolk, "IgE in the diagnosis and treatment of allergic disease," (in eng), J Allergy Clin Immunol, vol. 137, no. 6, pp. 1662-1670, Jun 2016, doi: 10.1016/j.jaci.2016.04.010.
J. Sánchez, A. Sánchez Biol, M. Múnera Biol, E. García, and J.-F. López, "Immunoglobulin E and G autoantibodies against eosinophil proteins in children and adults with asthma and healthy subjects," World Allergy Organization Journal, vol. 16, no. 2, p. 100742, 2023/02/01/ 2023, doi: https://doi.org/10.1016/j.waojou.2023.100742.
N. Rajvanshi, P. Kumar, and J. P. Goyal, "Global initiative for asthma guidelines 2024: an update," Indian pediatrics, vol. 61, no. 8, pp. 781-786, 2024.
L. Sun et al., "Difference of Serum Cytokine Profile in Allergic Asthma Patients According to Disease Severity," J Asthma Allergy, vol. 15, pp. 315-326, 2022, doi: 10.2147/JAA.S345759.
D. Russo, M. Lizzi, P. Di Filippo, S. Di Pillo, F. Chiarelli, and M. Attanasi, "Time-Specific Factors Influencing the Development of Asthma in Children," (in eng), Biomedicines, vol. 10, no. 4, Mar 24 2022, doi: 10.3390/biomedicines10040758.
A. H. Abbas, M. A. Rasheed, H. A.-A. Al-Hindy, M. J. Mousa, and H. A. A. Al-Shalah, "The role of serum IL-1β in combination with fractional exhaled nitric oxide in the diagnosis of adult bronchial asthma," NeuroQuantology, vol. 19, no. 8, pp. 13-9, 2021.
M. Patil et al., "Study of prevalence of Asthma in School Children in Pune and its relation with Family History of Asthma and Allergic disorders," Authorea Preprints, 2024.
Z. Liu, C. Niu, L. Ying, Q. Zhang, M. Long, and Z. Fu, "Exploration of the serum interleukin-17 and interleukin-27 expression levels in children with bronchial asthma and their correlation with indicators of lung function," Genetic Testing and Molecular Biomarkers, vol. 24, no. 1, pp. 10-16, 2020.
A. Jafarzadeh, M. Nemati, S. Jafarzadeh, P. Chauhan, and B. SAHA, "THE IMMUNOMODULATORY POTENTIALS OF INTERLEUKIN-27 IN AIRWAY ALLERGIES AND COVID-19," Authorea Preprints, 2020.
M. Xie et al., "IL-27 and type 2 immunity in asthmatic patients: association with severity, CXCL9, and signal transducer and activator of transcription signaling," Journal of Allergy and Clinical Immunology, vol. 135, no. 2, pp. 386-394. e5, 2015.
H. A.-A. M. Al-Hindy, A. J. H. Al-Athari, M. J. Mousa, S. J. Hameed, and S. H. Obeed, "The utility of serum IL-1β and CRP together with fractional exhaled nitric oxide in the diagnosis of asthma in adults," NeuroQuantology, vol. 19, no. 8, pp. 119-24, 2021.
K. M. Mohany, Y. Gamal, and Y. F. A. Raheem, "Association of LPCAT1-rs8352 genetic variant with susceptibility and severity of pediatric bronchial asthma: a case-control study," BMC Pediatrics, vol. 25, no. 1, p. 68, 2025/01/27 2025, doi: 10.1186/s12887-025-05425-x.
S.-L. Cheng, "Immunologic Pathophysiology and Airway Remodeling Mechanism in Severe Asthma: Focused on IgE-Mediated Pathways," Diagnostics, vol. 11, no. 1, p. 83, 2021. [Online]. Available: https://www.mdpi.com/2075-4418/11/1/83.
M. S. Jebur and A. M. Saud, "Serum Levels of Total IgE and Interleukin-13 in a Sample of Allergic Asthma Patients in Baghdad," Iraqi Journal of Science, vol. 61, no. 12, pp. 3208-3214, 12/29 2020, doi: 10.24996/ijs.2020.61.12.8.
S. Shaban and S. Brakhas, "Significance of total and specific IgE in asthma of Iraqi adult patients," Tikrit Journal of Pure Science, vol. 26, pp. 1-7, 12/03 2022, doi: 10.25130/tjps.v26i1.91.
A. J. H. Al-Athari, A. K. Aldhalmi, and H. A.-A. M. Al-Hindy, "Association of High-Sensitivity C-Reactive Protein and Vitamin D with Bronchial Asthma," Open Access Macedonian Journal of Medical Sciences, vol. 10, no. B, pp. 931-936, 2022, doi: 10.3889/oamjms.2022.8795.
S. Shareef, "Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity," Annals of Tropical Medicine and Public Health, vol. 23, 01/01 2020, doi: 10.36295/ASRO.2020.231807.
Downloads
Published
License
Copyright (c) 2025 University of Thi-Qar Journal of Science
This work is licensed under a Creative Commons Attribution 4.0 International License.
