Assessment of IL-27 as a Predictive Biomarker for Immune Response in Asthmatic Patients in Diyala Governorate

Authors

  • Ahmed Salman Department of Medical Laboratory Techniques, College of Health and Medical Techniques, Middle Technical University
  • Hind Jaber Department of Medical Laboratory Techniques, College of Health and Medical Techniques, Middle Technical University
  • Ekhlas Saddam Department of Medical Laboratory Techniques, College of Health and Medical Techniques, Middle Technical University

DOI:

https://doi.org/10.32792/utq/utjsci/v12i1.1368

Keywords:

Allergy, Asthma, IL-27, CRP, Total IgE

Abstract

Asthma is a persistent inflammatory respiratory disease characterized by coughing, wheezing, and intermittent dyspnea, as well as a sensation of chest tightness. The relationship between interleukin-27 (IL-27) and asthma is not precisely known yet.  This study attempted to estimate the serum level of IL-27 and its relationship with immunological and inflammatory markers in patients suffering from asthma. The demographic characteristics of this study were analyzed statistically. The serum levels of IL-27 were evaluated by Enzyme-linked immunosorbent assay (ELISA). CRP and Total IgE were evaluated by Cobas C111 and Cobas E411, respectively. The study showed a highly significant difference in IL-27, CRP, and total IgE when we compared between the patient group (62.12 ± 16.58 pg/ml, 11.2 ± 6.29 mg/l, and 297.26 ± 86.6 IU/ml), and the healthy control group (39.32 ± 3.576 pg/ml, 2.49 ± 1.1 mg/l, and 93 ± 18.3 IU/ml) respectively. ROC analysis of the studied biomarkers showed high sensitivity and specificity. That observation of elevation in all studied biomarker levels in asthma patients indicates a strong evidence in the diagnosis of asthma, as well as in the differentiation between acute and chronic cases, and in determining the severity of asthma.

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2025-06-25

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How to Cite

Salman, A., Jaber, H., & Saddam, E. (2025). Assessment of IL-27 as a Predictive Biomarker for Immune Response in Asthmatic Patients in Diyala Governorate. University of Thi-Qar Journal of Science, 12(1), 165-169. https://doi.org/10.32792/utq/utjsci/v12i1.1368