Determination of the relationship between percentage of hypo-osmotic swelling test scores and antisperm antibody assay in infertile patients after IUI technique

Abstract

This study was devised to compare the results of hypo-osmotic swelling (HOS) test and
antisperm antibody assay (ASA) for normozoospermic men and infertile patients affected
by autoimmune infertility. Thirty semen samples were collected by masturbation and
prepared by direct layering technique for IUI procedure.
Hypo-osmotic swelling test was performed by mixing 0.1 ml of semen with 1.0 ml of a 150
mOsm/ L NaCl as a hypo-osmotic solution and direct immunobead assay were used to
determine the presence of ASA bound on the sperm surface. However, sperm
concentration, sperm motility, progressive sperm motility, normal sperm morphology
were evaluated according to standard World Health Organization (WHO) criteria and
subjected to HOS test and ASA assay. The sperm prepared and incubated for 30 minute
in 5% COat 37ºC after in vitro sperm preparation.
The results reported that the percentage of HOS test score in ASA positive (55.20 ± 7.1)
sperm samples was significantly lower than that noticed in ASA negative (73.32 ± 4.8)
sperm samples (P<000.1). As a result, eight clinical pregnancy rates were accounts for
normozoospermic men without ASA and normal HOS test after in vitro sperm activation
and IUI procedure. Therefore, it was concluded that the use of HOS test as a simple and
dependable test to identify successful pregnancy after intra-uterine insemination. Further
studies are suggested to assess the effect of ASA on sperm plasma functional integrity
after ICSI and IVF-ET.