Detection of Hepatits C Virus (HCV) by ELISA, RIBA and Reverse Transcriptase- Polymerase Chain Reaction (RT-PCR) Technique among Kidney dialysis patients in Nineveh Governorate/Iraq.

Abstract

Introduction: the silent disease (Hepatitis C virus) is considered as one of the major causes of chronic hepatitis and cirrhosis, hence the aims of study to detect Hepatitis C virus among hemodialysis and peritoneal dialysis in Nineveh Governorate/Iraq.
Patients and Methods: (80) blood samples were collected from dialysis patients 46 (57.5%) males, (42.5%) females at age (15-60) years, the average age (37.5) years, include (50) heamodialysis patients, (30) peritoneal patients and (100) person from health blood donors as a control group.
We used ELISA version 3.0 to detect anti-HCV and confirm the positive and equivocal results by
Recombinant Immunoblot Assay version 3.0 (RIBA HCV 3.0), and used Reverse Transcribtase Polymerase chain reaction (RT-PCR) to confirm positive and equivocal results by RIBA technique.
Results: the current study showed significant differences (P≤0.05) between percentage of ant i-HCV and HCV RNA between heamodialysis, peritoneal dialysis and control group. The percentage of anti-HCV when tested by ELISA in serum of heamodialysis patients was (48%) positive, (48%) negative, (4%) equivocal, while (2%) positive (98%) in serum of peritoneal dialysis, (1%) positive, (99%) negative in control group. We used RIBA technique to confirm positive and equivocal results in ELISA the results showed (44%) positive, (52%) negative and (4%) equivocal between heamodialysis patients, (2%) positive, (98%) negative between peritoneal dialysis, while showed (1%) positive, (99%) between control group.